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cAMP (Cyclic Adenosine Monophosphate) ELISA Kit

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Description

cAMP (Cyclic Adenosine Monophosphate) ELISA KitThe cAMP (Cyclic Adenosine Monophosphate) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with cAMP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to cAMP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The

The cAMP (Cyclic Adenosine Monophosphate) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to cAMP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 85813252721

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